The overall goal of my research is to study the expression of the human UDP-glucuronosyltransferase 2B17 (UGT2B17) gene and investigate it as a possible new biomarker for human prostate cancer. This research is part of the Cancer Research Partnership between the JLC-Biomedical/Biotechnology Research Institute and the Lineberger Comprehensive Cancer Center at UNC-Chapel Hill. Towards this goal, we will examine the role growth factors and hormones have on the transcriptional activity of the UGT2B17 gene promoter region and identify single nucleotide polymorphisms (SNPs). In my lab, we will study the transcriptional activity of the UGT2B17 gene promoter by observing changes in DNA methylation and transient reporter gene activity in human cancer cell line systems.

Hatada, S., D.J. Grant and N. Maeda. 2003. An Intronic endogenous retrovirus-like sequence reduces human haptoglobin-related gene expression by affecting splicing. Gene. 319:55-63.

Gernon, A., Woldu, E., Godlevski, M.,Wilson, W., Gilmore, R. C., Grant, D. J., Chatterjee, P. K. and Kephart, D. 2003 Automated Purification of Dye Terminator Sequencing Reactions: an Approach to High Throughput Capillary Electrophoresis Sequencing of Large Templates. Journal of the Association of Laboratory Automation 8:19-23.

Grant, D. J., Hall, I. J., Eastmond, D. A., Jones, I. M., Bell, D. A. 2004. Bilirubin UDP-Glucuronosyltransferase 1A1 (UGT1A1) gene promoter polymorphisms and HPRT, glycophorin A, and micronuclei mutant frequencies in human blood. In press: Mutation Research.

Peters, U., Sinha, R., Bell, D. A., Rothman, N., Grant, D. J., Watson, M. A., Kulldorff, M., Brooks, L. R.,Warren, S. H., and DeMarini, D. M. 2004. Urinary Mutagenesis and Red Meat Intake: Influence of Cooking Temperature, Phenotype, and Genotype of Metabolizing Enzymes in a Controlled Feeding Study. In press: Environmental and Molecular Mutagenesis

Carreon, T, A.M., P.A. Schulte, R.B. Hayes, N. Rothman, G.K. Lemasters, D.J. Grant, R. Boissy, D.A. Bell, F.F. Kadlubar, G.P. Hemstreet, S. Yin, G. Li, and P. Leng. 2001. Congress of Epidemiology.

Grant, D. J. and D. Bell. The Bilirubin UDP-gluruconosyltransferase 1A1 Gene Polymorphisms: A Genetic Susceptibility Marker for Oxidative Damage and Cancer Risk? Mol. Carcin. 29: 198-204, 2000.

Teng, C.T., D.J. Grant, and M. Negishi. 1999. Ed. K. Shimazaki, Hokkaido, Japan, 18-22; May 1999. Elsevier Science, Netherlands, Excerpta Medica International Congress Series. 2000 Elsevier Science B. V., Shiimazaki et al., ed

Grant, D.J., H. Shi, and C.T. Teng. 1999. J. Mole. Endocrin. 23: 45-55.

Grant, D.J. and N. Maeda. 1993. Am. J. Hum. Genet. 52: 974-980.